The purpose of this study was to quantify the serum blood biomarkers of the inflammatory process induced by subcutaneous injection of 0.1% Carrageenan solution into the rat paw. There are a variety of endogenous compounds participate in the process of inflammation, which can be divide into two groups: pro-inflammatory (inducing inflammatory reaction) and anti-inflammatory (concluding inflammation). Over time, their ratio may vary. Analysis of the rat blood serum metabolome after 3 and 24 hours (n=3) after the onset of the inflammatory reaction was carried out using HPLC/MS/MS equipment (Shimadzu 8040), Lipid Mediators v.2 and SPE (Chromabond HR-X 60 mg cartridges). From the obtained profile of metabolites, the prostaglandin groups of 8-iso-PGE2, PGE2, PGD2 (pro-inflammatory) and 8-iso-PGA2, PGA2, PGJ2 (anti-inflammatory) were chosen. A change in the ratio of these molecules during growth and resolution of inflammation in the Carrageenan-induced edema of the rat paw has been established. Quantity of the selected metabolites was expressed using relative units (RU) through normalization 8-iso-PGE2, PGE2, PGD2 to PGE2-d4 and 8-iso-PGA2, PGA2, PGJ2 to PGA2-d4. Control values in rats without inflammation were 48.57 RU and 8.03 RU for the sum of 8-iso-PGE2, PGE2, PGD2 and the sum of 8-iso-PGA2, PGA2, PGJ2, respectively. 3 hours after the onset of inflammation, the amount of pro-inflammatory metabolites was 135.22 RU, while for 24 hours 91.72 RU. For the anti-inflammatory metabolites group, 10.18 RU was determined for 3 hours, and 10.34 RU for 24 hours. Thus, there was an increase and a subsequent decrease in the number of pro-inflammatory prostaglandins, whereas the amount of anti-inflammatory drugs by 24 hours remained high. An analysis of the ratio of pro- and anti-inflammatory mediators in vivo using the model of Carrageenan-induced edema of the rat paw can be used to compare the anti-inflammatory activity of potential drugs. The determination of the metabolic profile kinetics during inflammatory process makes it possible to clearly demonstrate inhibition of the inflammatory cascade, and the prevention from transition acute form of inflammatory to chronic.

Familial Shar-Pei amyloidosis is an autoinflammatory systemic disease characterized by pathological synthesis of the fibrillary protein in the cells of the reticuloendothelial system, followed by amyloid formation. The purpose of our research is mainly focused on the investigation of specific structural histological changes in kidneys, liver, and spleen of the Shar-Pei dogs suffering from familial amyloidosis. Materials and Methods: The studies included autopsy and post-mortem examination of the Shar-Pei dogs with the presumptive diagnosis of familial amyloidosis or other diagnoses. Samples of kidney, liver, and spleen tissues of all cadavers were collected for histological examination. Results: Our studies showed that amyloid was formed within the ground substance of the connective tissue. Early amyloid deposits were observed in the spleen samples, providing the pathomorphological marker of the initial stage of the process generalization, whereas during the later stages, amyloid was found in kidneys, liver, and myocardium. Gradually increasing amyloid deposits lead to compression and atrophy of the parenchymal cells, sclerosis, and multiple organ dysfunction syndrome, manifesting as a wide range of clinical signs. Discussion: As a result of the conducted post-mortem examination, we have revealed systemic amyloidosis in the cadavers of the animals, initially admitted with various pathologies, which proves the importance and relevance of timely diagnostics, detection of clinical manifestations, and latent forms of the condition. Histological examination is one of the most accurate diagnostic methods for this pathology.

The lack of trace elements, including selenium, affects the metabolism of minks and the process of forming high-quality coat. Aim: To identify the relationship between metabolic processes and formation of coat by application of selenium preparations of inorganic nature in minks.

Difficulties in the early stage detection of erythrocytes’ microrheological abnormalities during the development of hypertension are connected with falling out of clinicians’ opinion of persons with first signs of this pathology. It dictates the necessity of experimental investigations’ fulfillment on laboratory animals with just developed hypertension in them. Eighty-seven of healthy male rats of Wistar line at the age of 2.5–3 months were taken into the investigation. Twenty-nine animals of them had experienced no impacts and composed the control group. Fifty-eight rats had hypertension developed by prescribing them cardio angionefo pathogenic semisynthetic diet. For the purpose of investigation biochemical, hematological, and statistical methods were used. As a result of hypertension, the rats turned out to have developed an increase of systolic and diastolic pressure. At regular exercise, on the treadmill, the rats were noted to have a gradual decrease of their values during 60 days of investigation to the normal level. During hypertension development lipids’ peroxidation activated in rats’ erythrocytes because the activity of their antioxidant protection weakened. When hypertension occurred in rats the erythrocytes-discocytes quantity in blood was found to have decreased. It was accompanied by an increase of reversibly and irreversibly changed erythrocytes quantity in the examined animals’ blood. When hypertension occurred in rats a quick rise of erythrocytes’ sum in aggregate was found and the rise in these aggregates’ quantity was due to lowering of free erythrocytes’ number. During experimental hypertension modeling, we noticed that there was a very early decrease in the quantity of erythrocytes-discocytes in the rat blood, and their level raised reversibly and irreversibly with the strengthening of their aggregative ability.