Information about the defended postgraduate students

Vyacheslav Goryachev
Country: Russia
Year of protection: 2015
Research topic: Functional characteristic of chronostructure of the cardio-vascular system in the dynamics of genetic hypertension
Annotation to the dissertation: In the present work some indices characterizing functional state of the cardio-vascular system under long-term hypertension were studied. As a target of research we used spontaneously hypertensive rats (SHR) and normotensive Wistar rats (control) in which we implanted (surgically) radio-transmitters monitoring BP, ECG and motion activity for several months continuously. The obtained data were processed with computer programs: Dataquest A.R.T.4.2 Gold (USA) и ChronosFit (Germany). It was found that maximal daily systolic BP significantly increased at the stable stage of hypertension; inhibition of the chronotropic function of the myocardium at the late terms of the experiment was also typical. It was shown that rhythmic characteristics of the investigated indices were gradually suppressed as hypertension was developing which was also accompanied by disorganization of their circadian profiles.

Anna Korshunova
Country: Russia
Year of protection: 2016
Research topic: Pathogenic features of cell death in myocardial alterations of different genesis
Annotation to the dissertation: In the present work the features of initiation of cardiomyocyte regulated cell death at the early stages of cardiac alteration of different etiology were studied. The experiments were performed on male rabbits in which we modeled acute ischemia of the left ventricle, hemodynamic overload of the left ventricle and a diffuse toxic damage of the myocardium. Мechanisms of cardiomyocyte autophagy and apoptosis were evaluated by means of morphological and immunohistochemical investigation of the myocardium. Cardiomyocyte autophagy was studied by estimation of protein BECN1 expression, apoptotic process in cardiomyocytes – by estimation of proapoptotic protein BAX and antiapoptotic protein BCL-2. It was found that mechanisms mediating realization of some types of cardiomyocyte regulated death may have different properties depending on the character of myocardial damage. It was shown that under acute myocardial ischemia the activity of BECN1-induced cardiomyocyte autophagy is increased and on the 1-st day there is also activation of mitochondrial pathway of cardiomyocyte apoptotic death. Acute toxic damage and acute overload of the left ventricle are accompanied by inhibition of cardiomyocyte autophagy.