Sergey Goryainov
Head of the High-Resolution Mass Spectrometry and NMR Spectroscopy Laboratory, Shared Research and Educational Center
RUDN University Deputy Head of the Quality Assurance Department,
"You always have to think."
2008
Graduated from the Faculty of Physics, Mathematics and Natural Sciences, Peoples’ Friendship University of Russia (now – RUDN University), specialist degree in “Chemistry”.
2006 - present
Researcher at the Laboratory of Spectral Studies of A.V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences.
2006 - present
Member of the All-Russian Mass Spectrometric Society.
2010
Completed master's degree programme in “Environmental Chemistry”, Faculty of Physics, Mathematics and Natural Sciences, Peoples’ Friendship University of Russia (now - RUDN University).
2009 - present
Head of the High-Resolution Mass Spectrometry and NMR Spectroscopy Laboratory, Shared Research and Educational Center, RUDN University (SREC RUDN).
2010 - 2014
Postgraduate studies at RUDN University (Ecological Faculty, Medical Faculty). In 2010, completed an internship at the factory of JEOL Ltd (Tokyo, Japan). Currently, works on the thesis for the degree of Candidate of Chemical Sciences.
2012 - 2015
Researcher at the Laboratory of Metabolomics and Proteomics of the Federal Research Center for Nutrition, Biotechnology and Food Safety.
2015 - present
Deputy Head of the Quality Assurance Department, Shared Research and Educational Center, RUDN University.
2017
Internship at the University of Turku, Finland.
2018
Internship at the factory of Shimadzu Corp, Kyoto, Japan.
Teaching
Gives lectures to RUDN students of Additional professional education programmes:
- “Mass spectrometry of organic compounds”,
- “Chromatographic methods of analysis”,
- “Spectral methods of analysis”.
Developed Additional professional education programmes using remote technologies:
- “Molecular mass spectrometry in the pharmacopoeial analysis of the authenticity and quality of medicines, plant raw materials and biologically active additives”,
- “Instrumental methods of pharmacopoeial analysis”.
Science
- Developed in co-authorship guidelines for measuring the mass fraction of synthetic phosphodiesterase type 5 inhibitors (tadalafil, vardenafil, and sildenafil) in dietary supplements by high-performance liquid chromatography with ultraviolet and mass spectrometric detection. The guidelines are intended for bodies and organizations of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, accredited testing laboratories (centers) that monitor the quality and safety of food raw materials, food products and biologically active food additives.
- Developed in co-authorship the KNOW-HOW “Composition and method for obtaining tablets for resorption with lipophilic fractions of Nigella sativa L.”. The invention relates to the chemical and pharmaceutical industry, namely, to the production of new dosage forms, and was created in order to expand the range of target dosage forms of domestic production.
- Developed the module “Molecular and isotope mass spectrometry in the pharmacopoeial analysis of the authenticity and quality of medicines”. The module is intended for students of specialized specialties “Pharmacy” and “Chemistry”, as well as specialists with higher education working in pharmaceutical, food, and chemical enterprises. The purpose of the module is to expand the knowledge of the theoretical foundations of mass spectrometry and the relationship of spectrum parameters with the structure of molecules for use in the development of methods and methodology for the identification and quantitative analysis of pharmaceutical substances and drugs at all stages of their life cycle - from creation to use.
- Developed in co-authorship the composition for the treatment of anal fissures in the form of a cream. When creating the cream of isosorbide dinitrate, a storage-stable form of the solute was obtained in a therapeutically significant concentration in a non-polar solvent - castor oil. Ricinoleic acid in the oil can, when applied topically, increase blood flow at the application site, which contributes to the therapeutic activity of this cream. The combination of components in the composition represents a completely new approach to the treatment of anal fissures.
https://yandex.ru/patents/doc/RU2595799C1_20160827 - Developed in co-authorship a felodipine-based composition for the treatment of anal fissures. The invention relates to medicine and chemical-pharmaceutical industry and ensures that no phase separation of the composition, no sticky feeling and an increase in therapeutic activity. The effectiveness of the proposed composition has been tested clinically. The composition was applied perianally, twice a day for fourteen days in patients with anal fissure. The use of the composition made it possible to completely eliminate the morphological changes characteristic of the anal fissure, that is, to achieve complete healing.
https://yandex.ru/patents/doc/RU2710742C1_20200110
Scientific interests
- Qualitative and quantitative analysis of complex multicomponent water-organic systems of various origins (chemistry, ecology, pharmacy, medicine, agronomy);
- Determination of the authenticity and safety of medicines, dietary supplements and food products by a combination of quantitative NMR spectroscopy on various nuclei and mass spectrometry;
- Metabolomic studies by means of quantitative NMR spectroscopy on different nuclei and mass spectrometry.
Functional phenotypes, which cells can acquire depending on the microenvironment, are currently the focus of investigations into new anti-inflammatory therapeutic approaches. Glial cells, microglia, and astrocytes are major participants in neuroinflammation, but their roles differ, as microglia are cells of mesodermal origin, while astrocytes are cells of ectodermal origin. The inflammatory phenotype of cells can be modulated by ω-6- and ω-3-polyunsaturated fatty acid-derived oxylipins, although data on changes in oxylipin profiles in different cell adaptations to pro- and anti-inflammatory stimuli are scarce. Our study aimed to compare UPLC-MS/MS-measured oxylipin profiles in various rat astrocyte adaptation states.
This work highlights the discovered in-situ analytical reaction between primary/secondary alcohols and nitrogenous bases (pyridine, quinoline) that involves the substitution of hydroxyl groups for nitrogen-containing charged species and proceeds in an ionization region of Direct Analysis in Real Time mass spectrometry (DART-MS) instrument at gas stream temperature of 150-450 °C. Resulted cations provide strong signals in mass spectra and this ensures high sensitivity of the analysis.
Hyaluronic acid (HA), a major glycosaminoglycan of the extracellular matrix, has cell signaling functions that are dependent on its molecular weight. Anti-inflammatory effects for high-molecular-weight (HMW) HA and pro-inflammatory effects for low-molecular-weight (LMW) HA effects were found for various myeloid cells, including microglia. Astrocytes are cells of ectodermal origin that play a pivotal role in brain inflammation, but the link between HA with different molecular weights and an inflammatory response in these cells is not clear. We tested the effects of LMW and HMW HA in rat primary astrocytes, stimulated with Poly:IC (PIC, TLR3 agonist) and lipopolysaccharide (LPS, TLR4 agonist).
A phenomenon of endotoxin tolerance where prior exposure of cells to minute amounts of lipopolysaccharide (LPS) causes them to become refractory to a subsequent high-amount endotoxin challenge is well described for innate immune cells such as monocytes/macrophages, but it is still obscure for brain cells.
Although many neurological and psychiatric disorders reveal clear sex-dependent variations, the molecular mechanism of this process is not clear enough. Astrocytes are involved in the response of neural tissue to injury and inflammation, produce steroid hormones, and sense steroid presence. To explore the hypothesis that astrocytes may participate in sex-mediated differences of inflammatory responses, we have examined whether male and female primary rat astrocytes show different responses to lipopolysaccharide (LPS) as a toll-like receptor 4 (TLR4) agonist.
Recently, the modulation of cellular inflammatory responses via endogenous regulators became a major focus of medically relevant investigations. Prostaglandins (PGs) are attractive regulatory molecules, but their synthesis and mechanisms of action in brain cells are still unclear. Astrocytes are involved in manifestation of neuropathology and their proliferation is an important part of astrogliosis, a cellular neuroinflammatory response. The aims of our study were to measure synthesis of PGs by astrocytes, and evaluate their influence on proliferation in combination with addition of inflammatory pathway inhibitors. With UPLC-MS/MS analysis we detected primary PGs (1410 ± 36 pg/mg PGE2, 344 ± 24 PGD2) and cyclopentenone PGs (cyPGs) (87 ± 17 15d-PGJ2, 308 ± 23 PGA2) in the extracellular medium after 24-h lipopolysaccharide (LPS) stimulation of astrocytes.