Vasily Vasiliev
Candidate of Chemical Sciences
Deputy Director of the Precision instrumental methods of analysis department “PRIMA” of Shared Research and Educational Center RUDN University,
We will soon get “from synthetics - to nature”.
2014
Graduated from the Faculty of Physical, Mathematical and Natural Sciences, Peoples’ Friendship University of Russia, specialty “Chemistry”.
2014-2017
Postgraduate student at Peoples’ Friendship University of Russia.
2014 - present
Deputy Director of the Precision instrumental methods of analysis department “PRIMA” of Shared Research and Educational Center RUDN University.
2018
Candidate thesis on “Quality control of medicines and objects of plant origin by NMR 1H spectroscopy without the use of standard samples” was defended. Specialty “Pharmaceutical Chemistry, and Pharmacognosy “.
Vasilyev V. G. gives lectures to students of additional vocational education programs - employees of pharmaceutical companies on “Nuclear magnetic resonance”.
Science
- New methods of quantitative analysis of drugs and biologically active additives by nuclear magnetic resonance (NMR) without the use of standard samples was developed.
- A new method for identification and determination of glycosides of flavonoids for preparations from the leaves of Ginkgo biloba without the use of standard samples by the method of NMR spectroscopy was presented.
- A possibility of adaptation of methods of identification and quantitative determination of biologically active substances in medication and medicinal raw materials of plant origin and herbal remedies on their basis to simple and economical low-field NMR spectrometers was proved.
- A method of identification and quantitative determination of the composition of oligopeptide medication by low-field NMR spectroscopy was developed.
Scientific interests
- Proof of the structure of organic compounds;
- Study of the structure of phenolic compounds of plant origin by NMR spectroscopy;
- Study of the application of quantitative NMR spectroscopy to complex organic mixtures of plant origin;
- Qualitative and quantitative analysis of medication, medicinal raw materials of plant origin and herbal remedies on their basis by the method of NMR spectroscopy.
The title compound, C33H35N3O5, is the product of the multicomponent condensation of 1-benzyl-4- ethoxycarbonylpiperidin-3-one with 1,5-bis(2-formylphenoxy)-3-oxapentane and ammonium acetate. The molecule comprises a pentacyclic system containing the aza-14-crown-4-ether macrocycle, tetrahydropyrimidine, tetrahydropyridine and two benzene rings. The aza-14-crown-4-ether ring adopts a bowl conformation with a dihedral angle of 62.37 (5)° between the benzene rings. The tetrahydropyrimidine ring has an envelope conformation with the chiral C atom as the flap, whereas the tetrahydropyridine ring adopts a distorted chair conformation. Two amino groups are involved in intramolecular N-H⋯O hydrogen bonds. In the crystal, weak C-H⋯O hydrogen bonds link the molecules into layers parallel to the ab plane.
An analysis of some popular antibiotics was carried out by quantitative 1H NM R spectroscopy and DART mass spectrometry without the use of reference standards.
The composition of terpen lactones and flavonol glycosides of commercial preparation series based on Ginkgo biloba extracts was investigated by 1H NMR spectroscopy. The content of individual terpen lactones was determined using DMSO-d6 and acetone-d6 solvents. The effect of the structure of flavonol glycosides on the signal of the hydroxyl proton at a position 5 of the ring A was examined. A new approach was proposed for semiquantitative determination of the total amount of flavonol glycosides by the integral intensity of this signal, which is a superposition of the singlets in the region of 12.5–12.65 ppm of individual flavonoids in DMSO-d6. Since the corresponding signals of aglycones (quercetin, kaempferol, isorhamnetin), which are minor components of the Ginkgo biloba extracts, appear separately in a slightly different region (12.45–12.48 ppm), the proposed method can also be used for detecting adulteration of Ginkgo biloba extracts by means of the addition into them of relatively cheap aglycones or rutin as well as for assessment of the content of flavonoids of similar structure in some types of plant raw materials.