RUDN Medical Institute – One more step on the way to fighting cardiologic diseases
Joint research of the Department of general pathology and pathologic physiology and the Department of biology and general genetics. Expression of Bax protein and morphological changes in the myocardium in experimental acute pressure overload of the left ventricle.
The study was carried out on male Chinchilla rabbits (3-3.5 kg) distributed into 4 groups, 4 per group: control (intact animals) and 3 experimental (rabbits with acute LV overload after 1, 3, and 5 days).
The animals were kept and handled in accordance with the Order No. 755 of the Ministry of Health of the USSR (August 12, 1977), and the European Convention for protection of Vertebrates Used in Experiments or with Other Research Purposes (Strasbourg, 1986). Pressure overload of LV was created surgically by narrowing grid. The proportion of equally distant points occupied by positively stained cardiomyocyte cytoplasm to the total count of points occupied by the cytoplasm was calculated.
Morphometric analysis of the myocardium was performed on histological sections stained with he- matoxylin and eosin. The content (vol%) of muscular fiber, cardiomyocyte nuclei, foci of destruction and infiltration, and volume of extracellular space were measured under a microscope at ×400 in 30 visual fields using Avtandilov grid. The nucleus/cytoplasm ratio, i.e. the ratio of cardiomyocyte nuclei to muscular fiber was calculated (in %).
Our previous study of LV cardiomyocyte apoptosis on an identical experimental model showed an increase in activities of effector caspase-3 and initiatory caspase-8 mediating realization of apoptotic cell death by the external pathway . The decrease in Bax protein expression detected in the present study indicated that initiation of apoptotic processes in LV cardiomyocytes in acute LV overload did not depend on the mitochondrial pathway and was caused mainly by the receptor-mediated signal mechanisms.
Hence, acute LV pressure overload is associated with lesser Bax expression protein in LV cardiomyocytes combined with manifest morphological changes in the myocardium most pronounced on day 5 of the process.
December 28, RUDN University Rector Oleg Yastrebov and QS World Merit Head of Partnerships Zoya Zaitseva signed an agreement on large-scale cooperation in achieving 17 sustainable development goals identified by the UN.
In a year, RUDN University rose from 43 to 31 place in the international QS University Rankings: Emerging Europe & Central Asia 2022. Peoples’ Friendship University of Russia showed the best results in international scientific relations, academic reputation and employer reputation categories.
RUDN has risen to the 28th position in the international ranking of environmentally friendly universities UI GreenMetric 2021 remaining the “greenest” university in Russia. This is the best result in the history of the university’s ranking participation since 2011. The list of the “greenest” universities was published on December 14. There are 956 universities from 80 countries, including 54 universities from Russia. The goal of the UI GreenMetric — assess universities according to campus development ecological sustainability and the energy-saving model of university management.