RUDN Univesity Biologists Prove the Effectiveness of Silver Nanoparticles Against Phytopathogens

RUDN Univesity Biologists Prove the Effectiveness of Silver Nanoparticles Against Phytopathogens

RUDN University biologists proved the effectiveness of biogenic metal nanoparticles against some fungi infecting plants. The nanoparticles are obtained in a safe way from strawberry leaf extract.

Phytopathogenic fungi Botrytis cinerea, Pilidium concavum and Pestalotia sp affect many plants: strawberries, nightshades, beans and others. RUDN University biologists have proposed a fundamentally new way to combat these phytopathogens. To do this, they used nanoparticles of metals, silver and copper. The nanoparticles are obtained by a green method based on strawberry leaf extract, without no harmful reagents. This is the first successful application of biogenic metal nanoparticles to combat these phytopathogens.

“For evaluation of antifungal activity of these NPs, three pathogenic fungi were selectd: Botrytis cinerea, as an unspecialized necrotrophic fungal pathogen that attacks over 200 different plant species, Pilidium concavum, which is an opportunistic pathogen that causes leaf spots and stem necrosis in a wide range of hosts, mainly on strawberry plants and Pestalotia sp., which is reported to be infectious for azalea leaves. There are just a few reports carried out on the last two fungi, and there is no report on the antifungal effect of nanoparticles on these fungi”, said Maryam Bayat, PhD student at RUDN University.

Scientists reported the synthesis of biogenic metal nanoparticles in previous works, and now they have presented their first experimental use against fungi. To do this, RUDN University biologists used two methods: he agar dilution method and the spore germination method. In the first case, they put a potential antifungal substance in the nutrient medium (agar) in different concentrations. Then a culture of the fungus is sown there followed by growth monitoring. In the second method, they place spores (cellular structures that fungi use for reproduction) in a nutrient medium with an antifungal substance.

Silver nanoparticles slowed the growth of B. Cinerea and P. Concavum by 28% and 65.4%, respectively, at 0.01% concentration. The mechanism of this antifungal action is not completely clear, but RUDN University biologists have suggested that silver nanoparticles form pores on the cell membrane of the fungus. It causes the death of the cell and inhibit the budding process. Germination of B. Cinerea spores was completely suppressed by a solution with 0.01% silver nanoparticles concentration. Copper nanoparticles turned out to be ineffective against fungi — they practically did not affect the growth of spores and fungi.

“According to the results, these nanoparticles have the potential to be used as an antimicrobial agent in antibacterial and antifungal remediation or as an additive in conventional formulations. Silver nanoparticles were found to be the more effective antimicrobial agent against all examined pathogens in comparison to copper nanoparticles”, said Meisam Zargar, PhD in agricultural sciences, professor at RUDN University.

The results are published in the Molecules

All news
20 Jan
RUDN University chemist creates nanocatalysts for vanillin synthesis

RUDN University chemist proposed a new method to create catalysts on a porous silicon matrix with metal nanoparticles. Efficient catalysts for organic reactions are obtained, for example, for the synthesis of vanillin, which is in demand in the food and perfume industry.

16 Jan
RUDN University Linguist: Intimidation and Military Rhetoric in the Media during the Pandemic Make People Pessimistic

When talking about COVID-19, television, newspapers, magazines, and social media turn to battle metaphors that make the fight against the pandemic feel like a war. Also, the coronavirus is often discussed in an excessively alarming and threatening tone. This problem is so acute that there is even the term for that — infodemia. It describes the panic in the media and social networks. A linguist of RUDN University studied how such a language affects the notions of people regarding COVID-19.

29 Dec 2022
RUDN University Ecologists Find a Flaw in Arable Soil Organic Matter Detection Using Optical Remote Sensing Data

Ecologists of the RUDN University showed that one of the methods used to detect the soil organic matter turns out to be ineffective after precipitation. Studies have shown that the impact of rain on the surface of arable soils leads to a decrease in the accuracy of modelling of organic matter content by 70%. The ecologists also suggested the way to compensate for this negative phenomenon and increase the accuracy of calculations to 84%.