Structural unit: Institute of Medicine.
The Laboratory is a modern platform for studying the human body’s microbiota: from identifying and characterizing the immunobiological properties of commensal bacteria to creating next-generation probiotic preparations.
Resources
The laboratory facilities enable using the following research methods:
- PCR
- RT-PCR
- ELISA
- Working with eukaryotic cells
- Identifying microorganisms by their metabolic profile (BioLog system)
Equipment Fleet
- Binder ED 240 Drying and Heating Oven (Germany)
- GK
100-3 Autoclave (Russia) - Being BJ-120TL Incubator (China)
- Being BPN-40RHP Air Jacket СО2 Incubator (China)
- Senova BS-3021 Benchtop Orbital Shaking Incubator (China)
- Microbial Identification Test Panel — BioLog GEN III Omnilog Plus ID System (USA)
- QuantGene 9600 Real-Time PCR Thermal Cycler (China)
- Scientz-F Lyophilization Dryer (China)
- Liston Model A1210 Water Distiller (Russia)
- Mini-PROTEAN Tetra System BioRad Electrophoresis Chamber (USA)
- Millipore 2.5 L cell system (Germany) and Amicon M2000 High Performance Ultra Filtration Cell controllers (USA)
Laboratory Team
- Develops study designs and improves existing protocols to achieve optimal results
- Uses culturomic, biochemical, molecular and genetic methods and cell-based technologies
- Conducts comparative data analysis using international databases (NCBI; Genebank)
Work Procedures: isolate bacteria, analyze their characteristics and propose effective modification.
Partners
- Microbiology and Virology Department of Institute of prevention medicine after named Z.P. Soloviev of N.I. Pirogov Russian National Research Medical University
- Culturomic studies of human microbiota
- Identifying and preserving commensal bacteria
- Studying the immunobiological properties of commensal bacteria using cell cultures
- Profiling bacteria by their antibiotic susceptibility spectrum
- Searching for alternative ways to overcome antibiotic resistance in bacteria
The laboratory team conducts research into the immunobiological properties of difficult-to-cultivate commensal bacteria in humans using cell cultures and heterogeneous spheroids; tries out methods for testing human microbiota to determine its immunobiological properties.
- Promising results: separating commensal bacteria by their pro- and anti-inflammatory profiles, creating a collection of microorganisms with known immunobiological properties.
- Application: selecting strategies for restoring and modulating microbiota for effective cancer treatment.