Structural unit: Institute of Medicine.
The laboratory develops innovative methods for treating and diagnosing various human diseases using the CRISPR/Cas9 genome editing system, as well as various delivery systems for genetic constructs.
Resources
The laboratory facilities enable the full range of molecular biology work to create genetically engineered constructs of CRISPR/Cas9 systems and their derivatives.
Equipment Fleet
Solution preparation and sterilization equipment:
- autoclave
- distiller
- ultrapure water production system
- ice generator
- water bath
- pH meter
- magnetic stirrer
Gel electrophoresis equipment:
- horizontal and vertical gel electrophoresis systems
- DC power supply
- transilluminator
- gel visualization system
PCR equipment:
- abacterial air boxes
- thermocyclers (including those with real-time signal detection)
- centrifuges
- dispensers
- solid-state thermostats
Equipment for purification and preparation of pseudoviral particles and extracellular vesicles:
- high-performance multifunctional floor-standing centrifuge
- shaker-incubators
The laboratory team solves a full range of problems for academic and industrial partners in genetic engineering and bioengineering.
Partners
- Pirogov Russian National Research Medical University of the Ministry of Health of the Russian Federation
- Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences
- Mental Health Research Center, Russian Academy of Sciences
- Genetic and bioengineering
- Methods for efficient delivery of ribonucleic acid complexes to target cells
- Development of new approaches in biomedicine
A possible way to optimize the CRISPR/Cas9 system for knocking out the CCR5 gene is proposed: packaging CRISPR/Cas9 ribonucleoprotein complexes into extracellular vesicles and creating cell lines of producers.
Promising result: an innovative method for combating HIV infection.