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The method of spectral identification of hips and vitamin collection

The method of spectral identification of hips and vitamin collection

(Zhilkina V.Yu., Marakhova A.I., Kopylov V.V., Zhiltsova N.V., Zhiltsov V.V., Stanishevsky Y.M.)

The invention relates to the chemical and pharmaceutical industry and to a method for the identification of medicinal plant substances - rose hips and vitamin collection № 1 by analyzing the spectral characteristics of alcohol extraction. The method of identification of hips and vitamin collection № 1 includes obtaining alcoholic extract using ethanol with a concentration of 95%, an aliquot of 5-8 ml is placed in a 25 ml volumetric flask, add 5-8 ml of acetic acid 30%, 8 ml of alcohol 2% solution of aluminum chloride, bring 95% ethanol to the mark (pH of the solution 1.6 ± 0.4) and remove the spectrum against the background of a reference solution consisting of 5-8 ml of alcohol extraction and 8 ml of acetic acid 30% brought to the mark 95% ethanol; at the same time, three absorption maxima are simultaneously detected on the absorption spectrum, corresponding to: phenol carboxylic acids at 311 nm for vitamin collection № 1 and 323 nm for rose hips; flavonoids at 411 nm for vitamin collection № 1 and 404 nm for rose hips; anthocyanins at 540 nm for vitamin collection № 1 and 526 nm for rosehips.

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