1
Soil Scientists from RUDN University Have Determined the Optimal Size of Soil Pores for the Activity of Microorganisms
Soil Scientists from RUDN University Have Determined the Optimal Size of Soil Pores for the Activity of Microorganisms
Soil scientists from RUDN University found that the most favorable conditions for microorganisms are created in soil pores with a size of 60 to 180 micrometers. To visualize the relationship between the enzymes that produce soil microorganisms and pore size, the researchers created a three-dimensional map of soil structure. To do this, they used soil 2D zymography and X-ray computed tomography (3D). The article is published in the leading soil science journal Soil Biology and Biochemistry.

Soil types differ from each other not only in their chemical properties, but also in their location, shape, and pore size. Although any soil has both small and large pores, their ratio may be different. Larger pores (from 100 micrometers) are filled with air and are located between the soil lumps, small pores (10-100 micrometers) inside the lumps are filled with water. The pores are home to colonies of microbes that are responsible for biochemical reactions associated with the circulation of carbon, phosphorus, nitrogen and other chemical elements. Microorganisms improve soil fertility and carbon sequestration, so understanding what parameters (including pore sizes) determine the ‘well-being’ of microorganisms will help farmers increase yield.

Previously, the distribution of enzymes that secrete soil microorganisms was associated only with the chemical and biological properties of the soil. However, the physical structure of the soil also affects beneficial microbial communities and, consequently, enzymes. For the first time, soil scientists from RUDN University found optimal parameters of soil pores for microbial communities. To do this, they examined the pores using soil 2D-zymography — a biochemical method for studying the activity of enzymes, and X-ray computed tomography.

Soil samples from five land-use systems were taken from plots prepared for experiments by The Kellogg Biological Station in Michigan (USA): soil on which only corn was grown (1); white rye (2); millet (3); hybrid poplar with grassy undergrowth, (4) and soil on which plant communities successively changed as a result of natural factors or human activity (5). There were no live roots in the samples, meaning all the enzymes are produced by microorganisms.

To determine the distribution and size of pores in soils, samples measuring 5-10 centimeters were scanned with X-rays. Enzyme activity was mapped using zymography. Special membranes with substrates were placed on the surface of the samples, which emit fluorescent products upon contact. These products display the activity of enzymes in ultraviolet light. We studied the enzymes involved in the turnover of carbon, nitrogen and phosphorus in the soil: cellobiohydrolase, xylonase, acid phosphatase, β-glucosidase, leucinaminopeptidase and N-acetylglucosaminidase. Combining zymography and computer microtomography data allowed creating 3D maps that visualize the size of soil pores and enzyme activity in an intact soil sample.

In all the studied soil samples, regardless of the type, the most enzymes (and therefore the highest activity of microorganisms) were observed in pores with a diameter of 60-180 micrometers. Therefore, pores of this size are an ideal habitat for soil microbes. In smaller or larger pores, the activity of all six enzymes was less. Whole microbial colonies are located in the pores of this size (60-180 microns), and the access of water and oxygen is optimal. In the pores of this size (60-180 microns), more small roots grow, which provides greater saturation of the soil with readily available carbon. Thus, the size of soil pores does affect the distribution of enzymes.

The experiment confirmed for the first time that the combination of these two methods is effective for studying enzymes. In the future, soil scientists plan to improve the resolution of images and take into account additional factors such as fresh carbon intake, soil moisture and acidity. This will provide more accurate data and strengthen understanding of biochemical processes in situ — in the place where they occur, without any interference.

Article in Soil Biology and Biochemistry

Visiting Professors View all
03 Nov 2017
Michele Pagano is a graduate of the University of Pisa, a leading scientist, the author of more than 200 publications in international journals, and a participant in many international research projects
1014
International scientific cooperation View all
03 Nov 2017
The main goal of the RUDN University and UNISDR Office for Northeast Asia and Global Education and Training Institute for Disaster Risk Reduction at Incheon (UNISDR ONEA-GETI) cooperation is to obtain knowledge about disaster risk reduction and international experience in this area for creating training courses for basic and additional professional education in RUDN
506
Similar newsletter View all
20 Jul
RUDN chemist synthesized gold-based electrocatalysts

A RUDN chemist has synthesized an electrocatalyst based on gold nanoparticles with organic ligands, which can trigger both hydrogen production reactions and oxygen reduction reactions in fuel cells. The yield of products with the new catalyst was twice as high as when using a traditional platinum-based catalyst.

64
20 Jul
RUDN University chemist discovered a way to quadruple the speed of toluene photooxidation

A chemist from RUDN University has proposed a new way to control toluene photooxidation using composite catalysts — artificial diamond and titanium dioxide. The discovery refutes the previously existing physical hypothesis about the process of photooxidation of toluene.

70
20 Jul
RUDN University biologist developed new model for analyzing photosynthesis in vivo

A RUDN University biologist has developed a model for the analysis of photosynthesis in vivo. This method allows you to calculate the absorption coefficient of light by chlorophyll, based on its reflectivity. Analysis of light absorption is important for assessing ecosystem productivity, which affects the state of the biosphere and the global climate.

56
Similar newsletter View all