Mikhail Blagonravov

Research and Innovation

RUDN Scientists

Mikhail Blagonravov

Doctor of medicine

Associate Professor, department of General Pathology and Pathological Physiology named after Frolov V.А.

In process of scientific search not lose ability to doubt always.

+7 (495) 434-95-24 ext.: 1924

blagonravov-ml@rudn.ru

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Scientific interests:

myocardium cardiomyocyte programmed cell death apoptosis autophagy hypertension biological rhythms chronobiology telemetry monitoring melatonin epiphysis

2000

Graduated from the Peoples’ Friendship University of Russian, Medical Faculty, specialty “General Medicine”.

2005

Candidate of Medicine, Candidate thesis on “Pathophysiological mechanisms of the influence of the calcium antagonist verapamil on morphological and functional state of myocardium in the stage of development of compensatory hypertrophy” was defended.

2005 - 2016

Assistant, from 2009 – associate professor, from 2013 - professor of the Department of General Pathology and Pathological Physiology, the Institute of Medicine RUDN.

2008 - present

Head of the Scientific and Educational Laboratory of functional research methods, chronobiology and chronocardiology of RUDN.

2011

Doctor of Medicine, Doctoral thesis on “Apoptosis of cardiomyocytes as a model reaction of the altered heart”.

2014 - present

Director of the direction of training of highly qualified personnel in postgraduate school 30.06.01 “Fundamental Medicine”.

2015 - present

Member of the editorial Board of the journal “Bulletin of RUDN. Series Medicine”.

2016

The academic title of Associate Professor on specialty “Pathological Physiology” was awarded.

2016 - present

Head of the Department of General Pathology and Pathological Physiology named after Frolov V. A. the Institute of Medicine RUDN University.

2017 - present

Deputy Chairman of the dissertation Council D 212.203.06 at RUDN (14.03.03 “Pathological Physiology”, 14.03.02 “Pathological Anatomy”).

2016 - present

Member of the editorial Board of the journal “Bulletin of experimental biology and medicine”.

2017

Full member of the International Higher Education Academy of Sciences (IHEAS) on the “Science of Man”.

2009

Gratitude of the Rector of RUDN University for the significant contribution to the implementation of RUDN National project “Education”.

2016

Diploma of the scientific Council of RUDN University for conscientious labour, considerable contribution in development of medical education in RUDN University and in connection with the 55th anniversary of the Medical Faculty.

Teaching

Conducts lectures and practical classes in Russian and English for 3rd year students of the Institute of Medicine RUDN University:

“Pathophysiology, clinical pathophysiology” (for specialty - “General Medicine”),
“Pathophysiology - pathophysiology of head and neck” (for specialty - “Dental Medicine”),
“Pathology” (for the specialty “Pharmaceutical Science”)
Provides training of highly qualified personnel (in postgraduate school) in medical Sciences in the specialty 14.03.03 “Pathological Physiology”.

The author of the following course-books and study guides:

Frolov V. A., Blagonravov M. L., Demurov E. A., Bilibin D. P., Velichko E. V. Particular pathophysiology: study guide. - Moscow: Practical medicine, 2017. - 264 p. (Stamp of FIED of the Ministry of Education and Science of the Russian Federation)
https://rus.logobook.ru/prod_show.php?object_uid=2220310
The study guide includes all the topics that are discussed in the section “General Pathophysiology”. The material covers all the key issues that form the basis of this area of medical knowledge. Each topic includes definitions of basic concepts, classifications, descriptions of etiology, mechanisms of development and outcomes of the studied pathological processes and a number of specific diseases. The study guide was written in accordance with the programs of disciplines developed to meet the requirements of the FSES. For University students studying in “General Medicine”, “Dental Medicine”, “Pharmaceutical Science”, residents of all profiles and postgraduate students studying in the directions of “Fundamental Medicine”, “Clinical Medicine”.
Frolov V. A., Blagonravov M. L., Demurov E. A., Bilibin D. P., Velichko E. V. General pathophysiology: study guide. - M.: Practical medicine, 2016. - 224 p.
https://www.ozon.ru/context/detail/id/137342306/#section-description
The study guide includes all the topics that are discussed in the section “Particular Pathophysiology”. The material covers all the key issues that form the basis of this area of medical knowledge. Each topic includes definitions of basic concepts, classifications, descriptions of etiology, mechanisms of development and outcomes of the studied pathological processes and a number of specific diseases. The study guide was written in accordance with the programs of disciplines developed to meet the requirements of the FSES. For University students studying in “General Medicine”, “Dental Medicine” and “Pharmaceutical Science”.
Blagonravov M. L., Frolov V. A. Non-coronary diseases of the myocardium: study guide. - M.: Practical medicine, 2016. - 96 p.
https://rus.logobook.ru/prod_show.php?object_uid=2217402
The study guide presents material on a number of issues included in the section “Particular Pathophysiology” on myocardial diseases, etiologically and pathogenetically unrelated to the pathology of the coronary arteries. In addition to the existing course-books on pathological physiology, this study guide is intended for more profound study of relevant topics. In addition to describing the key concepts, classifications, etiology and pathogenesis of noncoronarogenic myocardial diseases, each chapter provides the basics. The study guide was written in accordance with the programs of disciplines developed to meet the requirements of the FSES. For University students studying in “General Medicine”, “Dental Medicine” and “Pharmaceutical Science”, residents of all profiles and postgraduate students studying in the directions of “Fundamental Medicine”, “Clinical medicine”.

Science

the concept of programmed cell death as a model reaction of altered heart was developed. It was defined that cardiomyocyte apoptosis is a nonspecific cardiac response to injury that is mediated by various initial mechanisms and can be directed to the elimination of damaged and functionally defective cellular elements. Currently, this concept was developed in relation to the molecular mechanisms that provide protection of myocardial cells in the developing pathology of the heart.
the scientific group under the leadership of M. L. Blagonravov studies pathogenetic mechanisms of violations of the rhythmic organization of the functions of the cardiovascular system, including using a unique technology of 24-hour telemetry monitoring of physiological functions. In particular, the influence of different types of light regime on the state of biological rhythms of the cardiovascular system in hypertension is studied. It has been shown that with a pronounced deficit of visible light in the chrono-structure of the heart rate there are changes that can limit the adaptive reserves of the body. On the other hand, excessive exposure to visible light has a more pronounced and less favorable effect on the body in hypertension.

Scientific interests

Pathophysiology of the cardiovascular system,
Programmed cell death,
Chronobiology,
Chronomedicine.

Blagonravov M.L., Medvedeva E.V., Bryk A.A., Goryachev V.A., Rabinovich A.E., Letoshneva A.S., Demurov E.A. 24-hour profile of blood pressure, heart rate, excretion of electrolytes, and locomotor activity in Wistar-Kyoto and SHR rats under under conditions of free-run rhythm // Bulletin of Experimental Biology and Medicine. – 2018.–Vol.166. – Issue 2. – 192-196.

We presented the results of our study of chronostructure of BP, HR, electrolyte excretion, and locomotor activity under conditions of “free-run rhythm” (light deprivation). In adult male Wistar-Kyoto (normotensive) and SHR (spontaneously hypertensive) rats, BP, biopotentials of the heart (ECG), and locomotor activity were recorded over 24 h by telemetric monitoring and the rate of excretion of electrolytes (Na+, K+, Ca2+, and Mg2+) during the nighttime and daytime hours was measured. It was found that under free-run rhythm, 24h profiles of BP, HR, excretory function of the kidneys, and locomotor activity underwent more considerable changes in normotensive Wistar-Kyoto rats in comparison with hypertensive SHR rats. However, hypertensive rats demonstrated pronounced changes in rhythmic characteristics of HR, which can restrict adaptation reserves of the cardiovascular system.

Blagonravov M.L., Bryk A.A., Medvedeva E.V., Goryachev V.A., Chibisov S.M., Syatkin S.P., Azova M.M., Zotova T.Yu. The effect of bright light on blood pressure and heart rate in essential hypertension // Neuropsychobiology: 30-th Annual Meeting of the Society for Light Treatment and Biological Rhythms, Groningen (The Netherlands), June 21-24, 2018. – 2017. – Vol. 76. – N 1. – P. 7.

In this study It was found that SBP and DBP were significantly higher in the SHR rats compared to the normotensive rats for the whole 1 hour period of BL exposure in comparison with the same time interval (from 10.00 to 11.00 am) of the previous day when BL was not used. The average levels of daytime (10.00 am – 7.00 pm) SBP and DBP were significantly increased in the hypertensive rats compared with the control rats which might indicate that the effect of BL remains notable even after the end of its exposure. For HR only a clearly defined tendency to an increase was seen. For the nighttime period after the day of BL exposure all of the monitored parameters of the hypertensive rats had the same values as in the controls. In normotensive rats the action of BL induced no significant changes in BP or HR. Our data shows that BL induces an increase in BP in hypertensive but not in normotensive rats both at the time of BL exposure and after it. This should be taken into account when using BLT in case of concomitant hypertension.

Ozheredov V.A., Chibisov S.M., Blagonravov M.L., Khodorovich N.A., Demurov E.A., Goryachev V.A., Kharlitskaya E.V., Eremina I.S., Meladze Z.A. Influence of geomagnetic activity and earth weather changes on heart rate and blood pressure in young and healthy population // International Journal of Biometeorology. – 2017. – Vol. 61. Issue 5. - P. 921-929

There are many references in the literature related to connection between the space weather and the state of human organism. The search of external factors influence on humans is a multi-factor problem and it is well known that humans have a meteo-sensitivity. A direct problem of finding the earth weather conditions, under which the space weather manifests itself most strongly, is discussed in the present work for the first time in the helio-biology. From a formal point of view, this problem requires identification of subset (magnetobiotropic region) in three-dimensional earth’s weather parameters such as pressure, temperature, and humidity, corresponding to the days when the human body is the most sensitive to changes in the geomagnetic field variations and when it reacts by statistically significant increase (or decrease) of a particular physiological parameter. This formulation defines the optimization of the problem, and the solution of the latter is not possible without the involvement of powerful metaheuristic methods of searching. Using the algorithm of differential evolution, we prove the existence of magnetobiotropic regions in the earth’s weather parameters, which exhibit magneto-sensitivity of systolic, diastolic blood pressure, and heart rate of healthy young subjects for three weather areas (combinations of atmospheric temperature, pressure, and humidity). The maximum value of the correlation confidence for the measurements attributable to the days of the weather conditions that fall into each of three magnetobiotropic areas is an order of 0.006, that is almost 10 times less than the confidence, equal to 0.05, accepted in many helio-biological research.

Blagonravov M.L., Bryk A.A., Medvedeva E.V., Goryachev V.A., Korshunova A.Yu., Azova M.M., Chibisov S.M., Syatkin S.P., Smirnova A.V., Varvanina G.G. Melatonin production in essential hypertension under common and modified light schedules // Neuropsychobiology. – 2016. – Vol. 74. – N 4. – P. 230.

It was found that daytime urinary concentration of 6-SMT was significantly lower in comparison with the nighttime values in hypertensive rats exposed to both 12:12 and 16:8 lightdark schedules. Daytime 6-SMT concentration was 25.5 ± 1.49 ng/ml in normotensive Wistar rats and it was significantly decreased to 16.27 ± 1.23 and 14.55 ± 1.32 ng/ml in SHR hypertensive rats kept under 12:12 and 16:8 light-dark schedules respectively. There was also an increase in the difference between day and night 6-SOMT in hypertensive rats kept under both light-dark regimens compared with controls. Meanwhile no difference in daytime 6-SMT urine contents was seen under light regimen 12:12 in comparison with 16:8 between the two groups of SHR rats. As regards nighttime, the values of urinary 6-SMT in Wistar rats, SHR rats under 12:12 and 16:8 light-dark regimens were as follows: 32.74 ± 2.78, 30.37 ± 2.54 and 26.08 ± 2.35 ng/ml with no significant differences between animal groups.

Blagonravov M.L., Korshunova A.Yu., Azova M.M., Bryk A.A., Frolov V.A. Expression of Bax protein and morphological changes in the myocardium in experimental acute pressure overload of the left ventricle // Bulletin of Experimental Biology and Medicine. – 2016. – Vol. 161. – No 2 – P. 312-315

The expression of Bax protein, marker of intracellular pathway of apoptosis initiation, in viable left ventricular cardiomyocytes and morphological changes in the myocardium in acute pressure overload of the left ventricle were studied in experiment on male rabbits. The content of Bax protein in the cardiomyocyte cytoplasm decreased, this indicating that the mitochondrial pathway was not involved in the realization of the apoptotic program. This decrease was associated with manifest destructive changes in the left ventricular myocardium.

Blagonravov M.L., Korshunova A.Yu., Azova M.M., Bondar’ S.A., Frolov V.A. Cardiomyocyte autophagia and morphological alterations in the left ventricular myocardium during acute focal ischemia // Bulletin of Experimental Biology and Medicine. – 2016. – Vol. 160. – No 3 – P. 398-400

In experiments on rabbits we evaluated the intensity of cardiomyocyte autophagia by the level beclin-1 protein and morphology of the left ventricular myocardium on days 1, 3, and 5 after the onset of focal ischemia caused by ligation of the descending branch of the left coronary artery. The morphological alterations in the left ventricular myocardium were accompanied by intensification of cardiomyocyte autophagia, which attained maximum on postligation day 1.

Blagonravov M.L., Frolov V.A., Goryachev V.A., Azova M.M. Features of the structure of the circadian rhythm of blood pressure and heart rate under genetically determined hypertension in the experiment // Bulletin of Experimental Biology and Medicine. – 2015. – Vol. 158 – No 6 – p. 723-725.

In SHR rats of different ages (22, 26, 30, 34, and 38 weeks), continuous 24-h telemetric monitoring of BP and HR was performed. The amplitude and power of oscillations of diastolic BP significantly decreased at the later stages of arterial hypertension (38 weeks), which was considered as a poor prognostic marker. We also observed a significant decrease in the mean daytime, nighttime, and maximum HR and mesor on weeks 30 and 34, but not on week 38, which can reflect triggering of the adaptive response followed by its exhaustion.

Blagonravov M.L., Frolov V.A., Azova M.M., Goryachev V.A. Characteristics of circadian rhythm of blood pressure during long-term hypertension development in SHR rats. // Bulletin of Experimental Biology and Medicine. – 2013. – Vol. 155. – Issue 5. – p. 612-614

The specific features of circadian rhythm of BP were investigated in freely moving male SHR rats using telemetry monitoring technique. BP was recorded in the abdominal aorta according to 24-h/4-month schedule. The data were obtained from 22, 26, 30, 34, and 38-week-old animals. Normotensive Wistar rats (22 weeks) served as the control. It was found that the mean 24-h, daytime, and nighttime systolic and diastolic BP in hypertensive rats significantly surpassed the control throughout the observation period and practically did not change during prolonged hypertension. Some prognostically negative changes in the circadian rhythm of the basic hemodynamics system parameters appeared with time. For instance, the maximum 24-h systolic BP significantly increased in comparison with the initial level.

Information about the defended postgraduate students

Vyacheslav Goryachev
Country: Russia
Year of protection: 2015
Research topic: Functional characteristic of chronostructure of the cardio-vascular system in the dynamics of genetic hypertension
Annotation to the dissertation: In the present work some indices characterizing functional state of the cardio-vascular system under long-term hypertension were studied. As a target of research we used spontaneously hypertensive rats (SHR) and normotensive Wistar rats (control) in which we implanted (surgically) radio-transmitters monitoring BP, ECG and motion activity for several months continuously. The obtained data were processed with computer programs: Dataquest A.R.T.4.2 Gold (USA) и ChronosFit (Germany). It was found that maximal daily systolic BP significantly increased at the stable stage of hypertension; inhibition of the chronotropic function of the myocardium at the late terms of the experiment was also typical. It was shown that rhythmic characteristics of the investigated indices were gradually suppressed as hypertension was developing which was also accompanied by disorganization of their circadian profiles.

Anna Korshunova
Country: Russia
Year of protection: 2016
Research topic: Pathogenic features of cell death in myocardial alterations of different genesis
Annotation to the dissertation: In the present work the features of initiation of cardiomyocyte regulated cell death at the early stages of cardiac alteration of different etiology were studied. The experiments were performed on male rabbits in which we modeled acute ischemia of the left ventricle, hemodynamic overload of the left ventricle and a diffuse toxic damage of the myocardium. Мechanisms of cardiomyocyte autophagy and apoptosis were evaluated by means of morphological and immunohistochemical investigation of the myocardium. Cardiomyocyte autophagy was studied by estimation of protein BECN1 expression, apoptotic process in cardiomyocytes – by estimation of proapoptotic protein BAX and antiapoptotic protein BCL-2. It was found that mechanisms mediating realization of some types of cardiomyocyte regulated death may have different properties depending on the character of myocardial damage. It was shown that under acute myocardial ischemia the activity of BECN1-induced cardiomyocyte autophagy is increased and on the 1-st day there is also activation of mitochondrial pathway of cardiomyocyte apoptotic death. Acute toxic damage and acute overload of the left ventricle are accompanied by inhibition of cardiomyocyte autophagy.