Tatyana Grebennikova

Research and Innovation

RUDN Scientists

Doctor of Biological Sciences

Professor, Department of Pharmaceutical and Toxicological Chemistry,

Knowledge of molecular and biochemical characteristics of the microorganism is the key to effective diagnostics and prevention of the disease, and therefore - to personalized medicine.

+7 (495) 434-70-01 ext.: 1926

grebennikova-tv@rudn.ru

Contact a scientist

Scientific interests:

reverse genetics phylogenetic analysis of genomes molecular epidemiology molecular Virology PCR and ELISA diagnostics personalized medicine influenza diagnostics rabies virus space biology diagnostics and prevention of viral diseases

1991

Graduated with honors from Lomonosov Moscow State University, the Biological Faculty the Department of Biochemistry.

1992 - 1995

Postgraduate studies in the Center for molecular diagnostics and treatment of the Ministry of health.

1995

Candidate thesis on “Isolation and purification of thermostable Tth DNA polymerase, study of properties and its use in methods of detection of RNA molecules” was defended, speciality “Biochemistry”.

2000 - 2006

Senior researcher of the laboratory of molecular diagnostics in D. I. Ivanovsky Institute of Virology.

2006

Doctoral thesis on “Molecular-genetic analysis and functionally significant genetic mutations of the virus of the reproductive and respiratory syndrome of swine” was defended, speciality “Virology”.

2006 - present

Head of laboratory of molecular diagnostics in D. I. Ivanovsky Institute of Virology, and then - in N. F. Gamaleya Federal Research Center for Epidemiology & Microbiology of the Ministry of health of Russia.

2007 - present

Professor of the Department of Pharmaceutical and Toxicological Chemistry, the Institute of Medicine RUDN University.

2009

The academic title of Associate Professor on specialty “Molecular biology” for the cycle of papers “Evolution of highly virulent H5N1 influenza virus in ecosystems of Northern Eurasia (2005-2009.)” was awarded, by D. I. Ivanovsky diploma of the Russian Academy of Medical Sciences for the best scientific work was awarded.

2010

The academic title of Professor of the Department of “Pharmaceutical and Toxicological Chemistry” was awarded.

2014 - present

Coordinator of the International European Scientific and Educational cluster “Baltic Networks against viral infections” of the project New Visby Network 2014-2016 “Towards healthy liver - the crossroads of viral hepatitis, alcohol use and life style patterns” Workpackage (WP2) “Epidemiology: detection and identification of liver pathogens” together with the Swedish Karolinska Institute.

2014 - present

Executive secretary of the editorial Board of the journal “Problems of Virology”.

2016

The academic title “Professor of Russian Academy of Sciences”, Department of Agricultural Sciences was awarded.

2016 - present

Member of the working group of RAS professors on monitoring the effectiveness of RAS professors and the Council on science and technology under the state Duma Committee on science and high technologies.

2017

The title of corresponding member of the RAS, Department of Medical Sciences (section of Biomedical Sciences) was awarded.

The supervisor and project manager in 8 projects of the ISTC and INTAS project. The coordinator of the International European Scientific and Educational cluster “Baltic Networks against viral infections”. Research projects were carried out jointly with groups of such organizations as: ARS, USDA: National Animal Disease Center (Ames, Iowa, USA), Plum Island Animal Disease Center (New York, NY, USA), Poultry Disease and Oncology Laboratory (East Lansing, Michigan, USA), South East Poultry Research Laboratory (Athens, Georgia, USA) and Central Veterinary Laboratory (UK), the New Visby Network 2014-2016 “Towards a healthy liver the crossroads of viral hepatitis, alcohol use and life style patterns” Workpackage (WP2) “Epidemiology: detection and identification of liver pathogens”. Maintains working contacts with such medical centers as the Center of Biotechnology of the University of Madrid, Karolinska Institute (Stockholm, Sweden), Medical University (Graz, Austria).
Participated in more than 30 international congresses, including conferences of the World Health Organization (WHO), the World Organisation for Animal Health (OIE), and FAO (Food and Agriculture Organization of the United Nations).

Teaching

Conducts laboratory and practical classes and lectures to graduates of the Institute of Medicine RUDN University on “Methods of molecular diagnostics in Pharmaceutical Chemistry”.

The author of the following course-books and study guides:

Pharmaceutical Chemistry Abramov A. U., Berlyand A. S., Garmonov S. Yu., Grebennikova T. V., Kalenikova E. I., Maksimova T. V., Morozova M. A., Pletneva T. V., Syroeshkin A.V., Uspenskaya, E. V., Shkrebneva I. I. M: Geotar Media. - 2017. - 816 p. Stamp Grif of the Ministry of Education and Science of the Russian Federation. ISBN 978-5-9704-4014-8
Abstract: The course-book outlines the basics of Pharmaceutical Chemistry in accordance with the program of discipline and the State standard for the speciality “Pharmacy”. The publication is intended for students of higher educational institutions studying in pharmaceutical, chemical-technological and other specialties, providing for the study of the course of Pharmaceutical Chemistry. The course-book can be recommended to postgraduate students of pharmaceutical specialties, as well as researchers and professors of the relevant departments.
https://istina.msu.ru/publications/book/31379140/
Biopharmaceutical Analysis, Pletneva T. V., Slatsky I. A., Grebennikova T. V., Akanina D. S., Garmonov S. Yu., Kalenikova E. I., Antipova N. V., Maksimova T. V., Uspenskaya, E. V. M., Publishing house MSU - 2017. - 123 p, ISBN: 978-5-00095-308-2
Abstract: The study guide “Biopharmaceutical analysis” is intended for students and masters of specialties “Pharmacy" and "Chemistry". The material is presented in two modules: “Biological methods of quality control of medicines” "and “Methods of evaluation of equivalence of medicines”. The study guide includes questions and tasks for seminars, examples of tests of control works.
https://search.rsl.ru/ru/record/01008873499

Science

More than 40 test-systems based on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) were developed, most were put into practice.
The intact DNA of terrestrial bacteria in cosmic dust of near-earth space was discovered together with professor Syroeshkin A.V.
The primary structure of the genomes of representatives of virus families Arteriviridae, Ortomyxoviridae, Rhabdoviridae. Flaviviridae was investigated and molecular genetic features of genomes and phylogenetic relationships for viruses circulating in the territory of the Russian Federation were defined.
Functional infectious genomes of RNA-containing viruses with the aim to identify genetic determinants of virulence for the development of diagnostic tools and prevention were created.
The test system based on PCR and sequencing to determine the marker mutations of sustainability of epidemic virus strains to antiviral drugs was developed. The pharmacopoeia article project was developed to determine the resistance of influenza A viruses to antiviral drugs.
Pharmacopoeia article projects for the diagnosis and typing of influenza A virus and М. Tuberculosis were developed.
Approaches to identify viral contamination in diagnostic and scientific laboratories and in clinics by methods of molecular diagnostics were developed.

Scientific interests

Molecular Epidemiology,
Molecular Virology,
Molecular Diagnostics,
Biochemistry,
Pharmaceutical Chemistry,
Space Biology.

Khametova K.M., Alekseev K.P., Yuzhakov A.G., Kostina L.V., Raev S.A., Musienko M.I., Mukhin A.N., Aliper T.I., Vorkunova G.K., Grebennikova T.V. Evaluation of the molecularbiological properties of human rotavirus A strain WA. Vopr Virusol. 2019;64(1):16-22. doi: 10.18821/0507-4088-2019-64-1-16-22.

The aim of this investigation was to study the biological and genetic properties of an actual epidemic human rotavirus A (RVA) strain Wa G1P[8] genotype. Human RVA G1P[8] Wa strain biological and molecular genetic properties were assessed in the process of the adaptation to MARC145 continuous cell line. Cell cultured RVA antigen was purified, concentrated and then characterized by the method of PAAG electrophoresis and immunoblot. To verify RVA Wa genome identity, electrophoresis of viral genomic RNA segments was performed. The lack of accumulation of changes in the RVA Wa genome during adaptation to various cell cultures and during serial passages was demonstrated by sequencing fragments of the viral genome.

Ilina, E.N., Solopova O.N., Balabashin D.S., Larina M.V., Aliev, Grebennikova T.V., Losich M.A., Zaykova O.N., Sveshnikov P.G., Dolgikh D.A. Generation and Characterization of a Neutralizing Monoclonal Antibody Against Rabies Virus RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY. Т.: 44, Вып.: 6, 2018, Стр.: 695-704. DOI: 10.1134/S1068162019010072

Rabies is a zoonotic disease, for which effective treatment methods after the onset of clinical symptoms have not been developed yet. Polyclonal sera, both human and equine, along with vaccines are important means of disease prophylaxis. However, due to adverse reactions to the immunoglobulins of animal origin, high cost, and limited availability of the safer human serum, polyclonal antibodies should be substituted for a stable and efficient preparation, which is recombinant neutralizing antirabies monoclonal antibodies (mAbs). This paper reports generation of the humanized mAb 1C5, which binds with the antigenic site (AS) III of the rabies virus glycoprotein (RABVG) and demonstrates high virus neutralization activity in the fluorescent antibody virus neutralization test, as a result of expression in the Chinese hamster ovary (CHO) cells.

Losich MA, Zaykova ON, Nepoklonova IV, Grebennikova TV, Verkhovsky OA, Odnovorov AI, Aliper TI. Molecular and biological characteristics of vaccinary ERA-CB 20M of rabies virus. Vopr Virusol. 2018;63(5):224-232. doi: 10.18821/0507-4088-2018-63-5-224-232. Russian.

The molecular and biological characteristics of the vaccine against rabies virus strain ERA-CB 20M obtained by the Russian rabiologist, doctor of medical sciences S.V. Gribencha by adapting and cloning the strain ERA and SAD in a transplantable BHK-21 C13 cell culture are presented. The spectrum of the most sensitive strain of rabies ERA-CB 20M cell lines was determined and the level of glycoprotein was quantitatively determined. Primary nucleotide sequences of fragments of the genome of the strain ERA-CB 20M (genes N and G) were obtained and phylogenetic analysis was carried out. Molecular analysis showed that this strain belongs to the group of vaccine strains SAD1. When compared with the reference strain SAD1, 10% of the nucleotide differences were revealed in the gene fragment N; 15%, in the gene fragment G.

Grebennikova TV, Syroeshkin AV, Shubralova EV, Eliseeva OV, Kostina LV, Kulikova NY, Latyshev OE, Morozova MA, Yuzhakov AG, Zlatskiy IA, Chichaeva MA, Tsygankov OS. The DNA of Bacteria of the World Ocean and the Earth in Cosmic Dust at the International Space Station. ScientificWorldJournal. 2018 Apr 18;2018:7360147. doi: 10.1155/2018/7360147.

Cosmic dust samples from the surface of the illuminator of the International Space Station (ISS) were collected by a crew member during his spacewalk. The sampler with tampon in a vacuum container was delivered to the Earth. Washouts from the tampon's material and the tampon itself were analyzed for the presence of bacterial DNA by the method of nested PCR with primers specific to DNA of the genus Mycobacteria, DNA of the strains of capsular bacteria Bacillus, and DNA encoding 16S ribosomal RNA. The results of amplification followed by sequencing and phylogenetic analysis indicated the presence of the bacteria of the genus Mycobacteria and the extreme bacterium of the genus Delftia in the samples of cosmic dust. It was shown that the DNA sequence of one of the bacteria of the genus Mycobacteria was genetically similar to that previously observed in superficial micro layer at the Barents and Kara seas' coastal zones. The presence of the wild land and marine bacteria DNA on the ISS suggests their possible transfer from the stratosphere into the ionosphere with the ascending branch of the global electric circuit. Alternatively, the wild land and marine bacteria as well as the ISS bacteria may all have an ultimate space origin.

Syroeshkin, A.V., Antipova, N.V., Zlatska, A.V., Zlatskiy, I.A., Skylska, M.D., Grebennikova, T.V., Goncharuk, V.V. The effect of the deuterium depleted water on the biological activity of the eukaryotic cells Journal of Trace Elements in Medicine and Biology. V. 50, 2018, P. 629-633

Here we show the dependence of the unicellular biosensor S.ambigua lifespan on the water D/H isotopic composition. This dependence is bell-shaped with descents both in case of deficiency or excess of deuterium in water. The influence of the water D/H isotopic composition on the cell culture proliferative potential and colony forming efficiency in vitro was tested on the human dermal fibroblasts. We observed that the deuterium depleted water stimulates cell colony formation at the early passages. The dynamics of the cell doubling index in the deuterium depleted water-based growth medium showed higher proliferation potential compared to the water with normal isotopic composition. Using scratch assay, we have also studied the impact of the growth medium D/H isotopic composition on the cell motility of human cancer cell lines A549 and HT29. We have shown that the deuterium depleted water considerably suppressed cancer cell lines amoeboid movement in vitro.

Grebennikova TV, Syroeshkin AV, Chichaeva MA, Esper SA, Lvov DK. Influenza A virus in the Western Arctic. Vopr Virusol. 2017;62(1):11-7

Small bays of bird bazaars of the Arctic Kola Peninsula (Barents Sea) have been studied. RNA of influenza A virus was found in the surface microlayer (SM) and aerosol samples from the bays located beneath bird colonies. The nucleotide sequencing of the PCR fragments from the SM and the sea aerosol showed their identity for each bay. Virus transfer mechanism along the “surface microlayer - sea aerosol” path has been proposed. The kinetic scheme of the virus-host-environment interaction, which allows the dependence of the viral population size on the temperature to be simulated, has been developed.

Zeberezhnyĭ AD, Grebennikova TV, Vorkunova GK, Yuzhakov AG, Kostina LV, Norkina SN, Aliper TI, Nepoklonov EA, Lvov DK. Engineering by reverse genetics and characterization of the new reassortant influenza virus strain H5N1 Vopr Virusol. 2014;59(6):23-7.

Reverse genetics was applied to engineering of the reassortantvaccine candidate strain against highly pathogenic avian influenza viruses (HPAIVs) of the H5 subtype. The new strain recPR8-H5N1 contains the HA gene from the Russian HPAIV A/Kurgan/05/2005 (H5N1), the NA and internal genes from A/Puerto Rico/8/34 (H1N1). The strain recPR8-H5N1 demonstrated the antigenic specificity (H5), high proliferation rate in 12 days chicken embryos, and was lethal for the embryos in 36 hours. An inactivated emulsified vaccine based on the strain recPR8-H5N1 elicited high antibody titers and protected 6-week-old chickens from lethal challenge with the HPAIV A/Kurgan/05/2005 (H5N1) on day 21 after single immunization. Infection of non-vaccinated birds with the strain recPR8-H5N1 did not cause any pathology, and the virus was not detected using PCR in blood and cloacal swabs on day 7 p.i. Specific weak seroconversion caused by infection with the strain recPR8-H5N1 was detected on day 14 p.i. As a result, a new influenza virus strain was obtained with modified properties.

Information about the defended postgraduate students

Suzanne Esper
Country: Russia
Year of protection: 2016
Research topic: Viruses on the surfaces and aerosoles
Annotation to the dissertation: The present study is dedicated to the research of possibility for viral nucleic acids detection (such as for viruses hepatitis A,C & E, adenoviruses, retroviruses, viruses from group A)on the surfaces and/or aerosoles for the determination of viral contamination at indoor spaces of different functionalities and to detection of new mechanisms for viral transmission in biocenosis.

Darya Akanina
Country: Russia
Year of protection: 2014
Research topic: Development of diagnostic tools for highly virulent virus strain influenza type A, subtype H5N1
Annotation to the dissertation: The research is dedicated to the development and improvement of laboratory detection methods for Influenza virus type A, subtype H5N1, and the antibodies of the last.

Marina Rotanov
Country: Russia
Year of protection: 2008
Research topic: Influenza type A virus strain sensitivity control to the antiviral pharmaceutical substances
Annotation to the dissertation: The research is dedicated to the development and standardization of diagnostic tests to antiviral drugs sensitivity control for virus strains Influenza type A, subtype H1 and H3, which have been eliminated from territory of Russian Federation (1995-2008 yy).

Nadejda Potapova
Country: Russia
Year of protection: 2006
Research topic: Real time test-system development on the basis of polymerase chain reaction for diagnostics of Tuberculosis
Annotation to the dissertation: The research is dedicated to the development and standardization of test-system for diagnostics of Tuberculosis on the basis of new modification of PCR-real time PCR method.