Stanishevsky Yaroslav Mikhailovich
Doctor of Chemistry
Professor, Director,
institute of biochemical technology and nanotechnology (IBCTN) of the RUDN
Already today do what others will only think about tomorrow!
1998
Graduated from the Faculty of Technology of Organic Substances of Lviv Polytechnic Institute. Specialty - “Biotechnology”.
1998 - 2001
Post-graduate student of Lomonosov Moscow State University of Fine Chemical Technologies (MITHT).
2001
Candidate thesis in two specialties: 03.00.23 - Biotechnology and 02.00.06 - High molecular compounds was presented. Academic degree - Candidate of Biological Sciences was awarded. Theme “Polymer disperse systems for medical and biological purposes”.
2003 - 2006
Doctoral student of D. Mendeleev University Chemical technology of Russian (MUCTR).
2005 - 2013
Assistant, associate professor, head of licensing and accreditation Department of Lomonosov Moscow State University of Fine Chemical Technologies (MITHT).
2012
Doctoral thesis in specialty 03.01.06 - Biotechnology, including bionanotechnology, was presented. Academic degree - Doctor of Chemical Sciences was awarded. Theme “Diagnostic test systems based on conjugates “polymer microsphere-bioligand” of medical and biological application”.
2013
The academic title of Associate Professor at the Department of Biomedical and Pharmaceutical Technologies was awarded.
2013
The main analyst of the Department for the Support of Accreditation Procedures of the FSBA National Accreditation Agency.
2013 - present
Director of the Scientific and Academic Center “Nanotechnology” IBTN RUDN.
2015 - present
Director of the Institute of Biochemical Technology and Nanotechnology RUDN (IBTN).
Teaching
- Conducts lectures and practical classes and seminars for students of master's and postgraduate studies of RUDN:
- “Biotechnology and Bionanotechnology”;
- “Nanotechnology in medicine”;
- “The use of polymers in biomedical technology and nanotechnology”;
- “Biochemical technology for BAC”.
- Leads three master's programs:
- “Biopharmaceutical technologies and management of pharmaceutical production” (direction “Industrial pharmacy”);
- “Innovative technologies and nanotechnology in medicine, pharmacy and biotechnology” (direction “Nanotechnology and microsystem technology”);
- “Biochemical technology and nanotechnology” (direction “Chemistry”.
- Leads the programs for training of highly qualified personnel (postgraduate study) “Biotechnology (including bionanotechnology)”.
- Leads the retraining program for specialists “Knowledge-intensive technologies and nanotechnology in medicine, pharmacy and biotechnology” and 2 programs of professional development training: “Principles of organization of technological and nanotechnology production in medicine and pharmacy”; and “Intellectual property management. Features of patenting in the field of knowledge-intensive technologies and nanotechnology”.
- The main implementer in the international project 511092-1-Tempus-2010-1-UK-JPCR on the development of a training master's program “Pharmaceutical engineering” in the direction “Biotechnology” (2011-2012).
The author of the following educational and methodical study guides:
- Atlas of medicinal plants and impurities to them. (Blinova O. L., Anisimova A. G., Stanishevskiy Ya. M., Pecherskaya L. G., Belonogova V. D. Marakhova A. I., Moscow: "GEOTAR-Media", 2018, p. 128). The study guide describes the morphological and anatomical features of Pharmacopoeia raw materials and closely related species (impurities), drawings, photographs and microphotography.
https://knigamir.com/catalog/nauki-i-obrazovanie_ID12/atlas-lekarstvennykh-rasteniy-i-primesey-k-nim-uchebnoe-posobie-o-l-blinova-i-dr-m-geotar-media-2018_ID1090039/ - Polymers. Physico-chemical properties, methods of production and methods of identification (Stanishevskiy Ya. M., Lobanov A. N., Lobanova N. A., Moscow: RUDN, 2016). The study guide describes the basic terms, concepts and definitions adopted in the field of chemistry of high-molecular compounds, characterized by the basic properties and distinctive features of polymers and oligomers. Methods of obtaining and identification of polymers and oligomers are described.
https://elibrary.ru/item.asp?id=27160996 - Laboratory workshop on working with the device “Nanophox particle size analyzer” (Zhilkina V.Yu., Stanishevskiy Ya. M., Marakhova A. I., Moscow: RUDN, 2016). The purpose of the educational and methodical study guide is to help students learn the principles of the NANOPHOX device. Practical recommendations contain the general method of operation on the device, information about the functions, measurement parameters when working with the device, as well as analysis of the data.
https://search.rsl.ru/ru/record/01008814070
Science
- The scientific bases of creation of highly specific and highly sensitive diagnostic test systems with the use of polymeric microspheres as carriers of bioligands for biomedical researches were introduced. Diagnostic test systems for early detection of markers (antigens, antibodies) of infectious and autoimmune human, mammal and bird diseases were developed.
- The technology of production of drug substance of nucleotide-peptide nature to create new domestic import-substituting drugs of immunostimulating action, as well as drugs for use in the treatment of type 2 diabetes was developed.
Scientific interests
- Synthesis of nanoparticles of silver, gold, selenium, carbon nanotubes, other elements and compounds for their use in medicine, catalysis and electronics. Study of physical and chemical characteristics of the obtained nanoobjects.
- Development of highly sensitive test systems operating at nano-and micro levels, and creation of diagnostic kits for early diagnosis of markers (antigens, antibodies) of infectious and autoimmune in human, mammal and bird diseases.
- Synthesis of biopharmaceutical medicines.
Thin films of copper(I) sulfide (Cu2S) are synthesized on a copper substrate by exposing it to vapor-phase sulfur-containing products resulting from the hydrothermal desulfurization of brown coal. The synthesized 0.1-mm-thick films have grain sizes in the range of 10‒20 μm, electrical resistivity ρ = 0.92 Ω cm at T = 300 K, and bang gap Eg = 1.91 eV. The roughness of the films, in terms of the arithmetic mean deviation of the assessed profile, is R a = 2.46 μm.
An energy-dispersive scheme using a bandpass filter based on pyrolytic graphite is proposed for determining the content of heavy elements from X-ray absorption spectra. The detected excitation spectrum is corrected to increase the accuracy of measurement. The initial shape of the photoabsorption jump is reconstructed in the approximation of an isolated atom by numerically solving the convolution equation.
The rudimentary stages with potential for development of developing diagnostic test systems for determining the presence of thyroglobulin in the blood of patients with thyroid problems are depicted in this research work. The presented development is supplemented by actual nanotechnologies for obtaining bionanoconjugates of gold nanoparticles with antibodies to antigen.
A method for the desulfurization of brown coal by treatment in supercritical water (T = 673.15 K, P = 30 MPa) with the subsequent deposition of gaseous sulfur compounds on a copper substrate was proposed. The products were refined fuel with a low heat value of 24 MJ/kg and Cu2S films with a thickness of 0.1 mm. The semiconductor Cu2S films can be used for the preparation of thin-film photoelectric energy converters. The greatest degree of the removal of sulfur from the brown coal was observed after an hour. The main sulfur-containing gas formed upon the autoclave treatment of brown coal in supercritical water was H2S.
An energy-dispersion scheme for determining the conсentrations of impurities of heavy elements from the absorption spectra in the regions of X-ray photoabsorption jumps is described. A semiconductor X-ray spectrometer and a pyrolytic graphite monochromator were used to record data in a spectral band of width up to 1 keV. The initial shape of the absorption spectrum in the approximation of an isolated atom was reconstructed by means of a numerical solution of the convolution equation. The scheme provides a sharp increase in the data acquisition and measurement sensitivity. The results of measurements of the Bi and Pb contents in samples with organic matrices and determination of the thicknesses of thin Mo films on diamond substrates are presented.
Rather than normal nucleoli, mammalian fully-grown (germinal vesicle, GV) oocytes and one-cell embryos (zygotes) contain intranuclear inclusions called "nucleolus-like bodies, NLBs" and "nucleolar precursor bodies, NPBs", respectively. Both entities remain almost inaccessible to various antibodies following standard procedure of immunofluorescence labeling that makes their protein composition and putative functions vague. Here we examine effects of molecular fixatives on the immunodetection of key nucleolar proteins in mouse NLBs and NPBs following different protocols of their fixation and post-fixation treatment. Our results show that the most impoverished for nucleolar proteins are zygotic NPBs, while only NSN-type NLBs contain all key nucleolar protein examined, including an rRNA processing factor SURF6/Rrp14. These observations support the idea that "nucleoli" of GV oocytes and zygotes have different capacities for ribosome biogenesis. The NSN-type NLBs can be involved in all nucleolar steps of ribosome production, including rDNA transcription, rRNA processing and pre-ribosome assembly. The NLBs of more mature SN-oocytes may only be capable of pre-ribosome assembly, whereas zygotic NPBs are, most likely, excluded from ribosome production.
The FDA Dissolution Database was reviewed using the following criteria: dosage forms, apparatus, rotation/pulsation speed, dissolution media, sampling time points, and trends for special dosage forms. In July 2015, there were 1084 drug products in the database, more than 50% thereof in tablet form. The paddle (Apparatus 2) is the most common apparatus in the database and is recommended for 488 products (45%). Rotation speeds listed in the database are 35–200 rpm for Apparatus 1 and 25–200 rpm for Apparatus 2. Deaerated or degassed water is recommended for 114 methods. The pH values for the most commonly cited dissolution media are in the range of 1–7.5; however, several dissolution methods have pH values that are out of physiological range (pH 12 for celecoxib capsules, pH 9.5 for glyburide tablets, pH 8.0 for Rabeprazole Sodium Tablets, pH 7.8 for Glimepiride Tablets).
The possibility of the efficient band reject filtration of the continuous X-ray excitation spectrum in the energy range E ≥ 8 keV is demonstrated. This makes it possible to strongly increase the sensitivity of energy dispersive X-ray spectroscopy at detecting of weak fluorescence lines. Spectral rejection is implemented by transmitting a primary beam through highly oriented pyrolytic graphite with given structural parameters. Diffraction extinction in pyrolytic graphite ensures the possibility of reducing the intensity by more than 20 dB and rejecting the spectral band with a width of ~1 keV. The reduction of statistical fluctuations of the background of elastically scattered radiation is achieved when the bottom of the formed spectral valley is adjusted to the analyzed fluorescence line. The proposed scheme of band reject filtration also allows the suppression of intense characteristic lines in the primary and scattered radiation spectra.
The salient technological properties of coal slurries are studied using fusel oil as the dispersion medium. The distinctive features of the rheological behavior and flow pattern of coal slurries as a function of the nature of coal are ascertained. It is shown that the effective viscosity of coal slurries decreases as the coal passes from the lignite to the anthracite stage. The calorific value and degree of combustion of coal slurries in fusel oil are higher than those of the original coal.
In this article, we have studied the influence of Si3N4 and SiO2 thin film gate dielectrics on the current-voltage characteristics of the graphene-based transistor. The test structure of graphene transistor was fabricated with the top and back gate. Graphene has been produced by chemical vapor deposition, and then transferred to the silicon dioxide on a silicon wafer. The channel of the transistor has been formed by etching in oxygen plasma through a photolithographic mask. Metals electrodes of the drain, source, and gate were deposited by resistive evaporation in a vacuum. It was used titanium / aluminum with a thickness of 50/200 nm. In the case of the back gate, silicon dioxide was used, obtained by thermal oxidation of the silicon substrate. For top gate was used silicon nitride deposited by plasma chemical deposition. It was demonstrated that field effect is more pronounced for the case of SiO2 back gate compare to the Si3N4 top gate. For the SiO2 back gate we have observed that the source- drain current decreases, from 2 mA to 3 mA, with increasing the gate voltage, from 0 to 40 V, at constant source-drain voltage, 2 V. In case of Si3N4 top gate the modulation of source-drain current was not significant for the comparable electric field strength. Based on the value of gate voltage for current minima in transfer function the poor quality of Si3N4 –graphene interface is concluded.